用相应的 ID 提取生物体计数?

用相应的 ID 提取生物体计数?

我有一个包含许多列的文件,例如:

ID1 XP_026389348.1_stearoyl-[acyl-carrier-protein]_9-desaturase,_chloroplastic_[Papaver_somniferum]
ID2 XP_026389348.1_stearoyl-[acyl-carrier-protein]_9-desaturase,_chloroplastic_[Papaver_somniferum]
ID3 XP_026389348.1_stearoyl-[acyl-carrier-protein]_9-desaturase,_chloroplastic_[Papaver_somniferum]
ID4 XP_026389348.1_stearoyl-[acyl-carrier-protein]_9-desaturase,_chloroplastic_[Papaver_somniferum]
ID5 XP_026389348.1_stearoyl-[acyl-carrier-protein]_9-desaturase,_chloroplastic_[Papaver_somniferum]
ID6 XP_022013305.1_60S_ribosomal_protein_L36-2-like_[Helianthus_annuus]
ID7 XP_022033863.1_60S_ribosomal_protein_L36-2-like_[Helianthus_annuus]
ID8 XP_022033864.1_60S_ribosomal_protein_L36-2-like_[Helianthus_annuus]
ID9 XP_022033865.1_60S_ribosomal_protein_L36-2-like_[Helianthus_annuus]
ID10    NP_850400.1_Plant_stearoyl-acyl-carrier-protein_desaturase_family_protein_[Arabidopsis_thaliana]
ID11    XP_015383392.1_60S_ribosomal_protein_L36-3-like_[Citrus_sinensis]
ID12    XP_015383392.1_60S_ribosomal_protein_L36-3-like_[Citrus_sinensis]
ID13    XP_019051818.1_PREDICTED:_stearoyl-[acyl-carrier-protein]_9-desaturase,_chloroplastic_isoform_X2_[Nelumbo_nucifera]
ID14    XP_019051818.1_PREDICTED:_stearoyl-[acyl-carrier-protein]_9-desaturase,_chloroplastic_isoform_X2_[Nelumbo_nucifera]
ID15    XP_019051818.1_PREDICTED:_stearoyl-[acyl-carrier-protein]_9-desaturase,_chloroplastic_isoform_X2_[Nelumbo_nucifera]
ID16    XP_021982111.1_stearoyl-[acyl-carrier-protein]_9-desaturase,_chloroplastic_[Helianthus_annuus]
ID17    NP_001150213.1_uncharacterized_protein_LOC100283843_[Zea_mays]
ID18    XP_027164486.1_stearoyl-[acyl-carrier-protein]_9-desaturase,_chloroplastic_[Coffea_eugenioides]
ID19    XP_009419937.1_PREDICTED:_60S_ribosomal_protein_L36-3-like_[Musa_acuminata]
ID20    XP_020267482.1_60S_ribosomal_protein_L36-2-like_[Asparagus_officinalis]

我想从 [ ] 中的第二列中提取生物体名称,并用它们各自的 ID 来计算它们,例如:

5   Papaver somniferum       ID1
                             ID2
                             ID3
                             ID4
                             ID5
4   Helianthus annuus        ID6
                             ID7
                             ID8
                             ID9
1   Arabidopsis thaliana     ID10
2   Citrus sinensis          ID11
                             ID12
3   Nelumbo nucifera         ID13
                             ID14
                             ID15
1   Helianthus annuus        ID16
1   Zea mays                 ID17
1   Coffea eugenioides       ID18
1   Musa acuminata           ID19
1   Asparagus officinalis    ID20

我尝试过一些事情:

cat file | cut -f2 | rev |awk -F "[" '{gsub("]", "");print $1 | "rev"}' | sed '/#/d' | sort |uniq -c| sort -nr

给出输出:

5   Papaver somniferum
4   Helianthus annuus
1   Arabidopsis thaliana
2   Citrus sinensis
3   Nelumbo nucifera
1   Helianthus annuus
1   Zea mays
1   Coffea eugenioides
1   Musa acuminata
1   Asparagus officinalis

谢谢。

答案1

$ datamash -t' ' -g2 count 1 collapse 1 < <(sed 's/^\(ID[0-9]*\).*\[\([^]]*\)\]$/\1 \2/' file) \
   | awk '{ print $2,$1,$3 }' \
   | sed 's/,\(ID[^,]*\)/\n# # \1/g' \
   | column -t \
   | tr '#' ' '
5  Papaver_somniferum     ID1
                          ID2
                          ID3
                          ID4
                          ID5
4  Helianthus_annuus      ID6
                          ID7
                          ID8
                          ID9
1  Arabidopsis_thaliana   ID10
2  Citrus_sinensis        ID11
                          ID12
3  Nelumbo_nucifera       ID13
                          ID14
                          ID15
1  Helianthus_annuus      ID16
1  Zea_mays               ID17
1  Coffea_eugenioides     ID18
1  Musa_acuminata         ID19
1  Asparagus_officinalis  ID20

步骤1:使用以下命令提取 ID 和生物体名称sed

$ sed 's/^\(ID[0-9]*\).*\[\([^]]*\)\]$/\1 \2/' file
ID1 Papaver_somniferum
ID2 Papaver_somniferum
ID3 Papaver_somniferum
ID4 Papaver_somniferum
ID5 Papaver_somniferum
ID6 Helianthus_annuus
ID7 Helianthus_annuus
...

第2步sed将 的输出输入 GNUdatamash并在第二个字段上分组,在第一个字段上计数并折叠:

$ datamash -t' ' -g2 count 1 collapse 1 < <(sed 's/^\(ID[0-9]*\).*\[\([^]]*\)\]$/\1 \2/' file)
Papaver_somniferum 5 ID1,ID2,ID3,ID4,ID5
Helianthus_annuus 4 ID6,ID7,ID8,ID9
Arabidopsis_thaliana 1 ID10
Citrus_sinensis 2 ID11,ID12
Nelumbo_nucifera 3 ID13,ID14,ID15
Helianthus_annuus 1 ID16
Zea_mays 1 ID17
Coffea_eugenioides 1 ID18
Musa_acuminata 1 ID19
Asparagus_officinalis 1 ID20

步骤3:添加一些格式以将输出转换为表格形式:

  • awk '{ print $2,$1,$3 }'交换第 2 列(计数)和第 1 列(生物体名称)
  • sed 's/,\(ID[^,]*\)/\n# # \1/g'将每个逗号和 ID 替换为换行符、两个空格分隔的虚拟字符#和 ID(使用 GNU sed
  • column -t格式为表格
  • tr '#' ' '#用空格替换虚拟字符

答案2

使用 GNUawk和 GNU column

awk -F'[][ ]*' '{print ($(NF-1)==n?OFS:$(NF-1)),$1; n=$(NF-1)}' OFS=, file \
 | awk -F, -v OFS=, 'NF==3{line=line RS $0;i++} NF==2{if(line)print i,line; line=$0; i=1}' \
 | column -t -n -s,

第一个 awk 命令提取并以该格式显示所需的字段:

Papaver_somniferum,ID1
,,ID2
,,ID3
...

第二个 awk 命令构建一个由所有同名字段组成的新行,并将其计数添加到前面。

最后,column 命令在选项的帮助下将结果显示为 3 列-n

5  Papaver_somniferum    ID1
                         ID2
                         ID3
                         ID4
                         ID5
4  Helianthus_annuus     ID6
                         ID7
...

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