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文本处理-perl

tag-icon • tag-icon text-processing awk perl columns
文本处理-perl

我是一名生物学家。我需要计算机专家的帮助来帮助编辑文本文件。

输入文件如下所示：

##dsfsd2
##sdf-sdf sasg 5.6.3
gi34_ex Gen  CDS     161     317     .       +       .       Name=Xm ZAK;created by=User
gi56_ex Gen  CDS     2194    2280    .       +       .       Name=Xm ZAK;created by=User
gi37_ex Gen  CDS     2848    2951    .       +       .       Name=Xm ZAK;created by=User
gi37_ex Gen  CDS     4554    4619    .       +       .       Name=Xm ZAK;created by=User
gi37_ex Gen  CDS     4729    4756    .       +       .       Name=Xm ZAK;created by=User
gi37_ex Gen  extracted region        1       11677   .       +       .       Name=Extracted region from gi|371443185|gb|JH556675.1|;Extracted interval="1960862 -> 1972538"

请注意，从第三行开始，输入有 12 列（$1 到 $12），制表符分隔。文件的最后一行有 16 列（$1 到 $16），制表符分隔。 ## 行应该被忽略。

我想将最后一行的 14 美元 (interval="1960862) 仅将数字 (1960862) 添加到 $4 列 (161,2194,2848..4729) 即 (161+1960862=1961023,2194+1960862=1963056.. ) 和 $5 列 (317,2280,2951..4756) 即 (317+1960862=1961179,2280+1960862=1963142)，忽略最后一行。

输出应如下所示：

##dsfsd2
##sdf-sdf sasg 5.6.3
gi34_ex Gen  CDS     1961023     1961179     .       +       .       Name=Xm ZAK;created by=User
gi56_ex Gen  CDS     1963056    1963142    .       +       .       Name=Xm ZAK;created by=User
gi37_ex Gen  CDS     1963710    1963813    .       +       .       Name=Xm ZAK;created by=User
gi37_ex Gen  CDS     1965416    1965481    .       +       .       Name=Xm ZAK;created by=User
gi37_ex Gen  CDS     1965591    1965618    .       +       .       Name=Xm ZAK;created by=User
gi37_ex Gen  extracted region        1       11677   .       +       .       Name=Extracted region from gi|371443185|gb|JH556675.1|;Extracted interval="1960862 -> 1972538"

答案1

这是一个可行的解决方案。你可以在评论中找到解释。

#!/usr/bin/perl
use warnings;
use strict;

open my $IN, '<', '1.in' or die $!;

my $line;
$line = $_ while <$IN>;                 # Remember the last line.
my $last = $.;                          # Remember the number of the last line.

my $interval = (split /\t/, $line)[13]; # Extract the 14th column.
$interval =~ s/[^0-9]+//;               # Keep only the number.

seek $IN, 0, 0;                         # Rewind to the beginning of the input.
$. = 0;                                 # Restart the line counter.
my $start = 1;                          # Flag to skip first lines.
while (<$IN>) {
    my @columns = split /\t/;
    /^##/ or undef $start;              # Unset start if the header is over.
    if (not ($start or $. == $last)) {  # Not header or last line?
        $_ += $interval for @columns[3, 4];
    }
    print join "\t", @columns;
}

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