我需要在页面底部放置一张全宽图片,在顶部放置一张小的半尺寸图片。中间会有文字。我试过摆弄这两张图片的h
、t
、b
,!
但无济于事。
这是我的代码:
%TC:ignore
\begin{figure}[!t]
\centering
\includegraphics[width=1\linewidth, height=5 cm]{Figures/iVSMCstaining.png}
\caption{\textbf{Staining confirming iVSMC generation.} VSMCs that were stained for smooth muscle aortic alpha-actin 2 (ACTA2) and 4′,6-diamidino-2-phenylindole (DAPI). Red corresponds to ACTA2 conjugation, and light blue corresponds to DAPI conjugation. Alexa-fluorophore 555 was the secondary antibody used to visualize the proteins of interest. Scale bar = 400 um.}
\label{fig:DriftCorrection}
\end{figure}
%TC:endignore
Given the qPCR analysis and staining results, it is apparent that our differentiation protocol resulted in the generation of iPSC-derived VSMCs.
\subsection*{E-protein constructs}
Two methods were used to design the final E-protein CRISPR delivery systems; the formation of in-del mutations via non-homolgous end joining (NHEJ) CRISPR editing and base editor CRISPR editing. Each method used a multi-vector approach, with both methods making use of a pSaGuide Cas9 plasmid that contained our guide sequence that helped target the CRISPR constructs to our gene of interest. The NHEJ method uses the guide plasmid in combination with a plasmid that expresses a catalytically active version of cas9. The base editor method combines the guide plasmid with a second plasmid that fuses a catalytically dead Cas9 to a cytidine deaminase. A multi-vector approach was used in order to avoid having plasmids that were too large, and therefore unable to enter the iPSCs.
%TC:ignore
\begin{figure*}[!h]
\centering
\includegraphics[width=\textwidth]{Figures/guideplasmiddesign.png}
\caption{\textbf{Guide Plasmid Design} \textbf{A)} The pSaGuide backbone was ordered from Addgene.The pSaGuide plasmid contains an ampicillin resistance (AmpR) cassette, a kanamycin/neomycin resistance (KanR/NeoR) cassette, a U6 promoter region, and the Sa gRNA scaffold. \textbf{B)} The chart in the top corner represents the guide RNA oligonucleotide (oligos) sequences that were designed and ordered as well. The oligos were annealed together to form double stranded DNA (dsDNA) and phosphorylated to convert the deoxyribonucleotide diphosphates (DNDPs) to deoxyribonucleotide triphosphates (DNTPs). The conversion from DNDP's to DNTP's allows the DNA to get ligated into the cut pSaGuide backbone via the use of overhanging sticky ends. Each oligo has a sequence that is analogous to the ends of the cut plasmid, which allows for easy ligation and incorporation.}
\label{fig:GeneralDiagram}
\end{figure*}
%TC:endignore
答案1
对于文档类,我选择了第一个尝试过的方法。由于您没有提供 MWE,因此不能保证它也对您有用。如果它没有帮助,我很乐意删除此帖子,但如果您提供 MWE,这可以说是更好地利用我们的时间。
\documentclass{article}
\usepackage{wrapfig}
\usepackage{graphicx}
\usepackage{lipsum}
\begin{document}
\lipsum[1-4]
\clearpage
\begin{wrapfigure}[13]{r}[10pt]{0.5\linewidth}
\centering
\includegraphics[width=0.9\linewidth]{example-image-a}
\caption{Pfft.}
\label{fig:Pfft1}
\end{wrapfigure}
\begin{figure}[!b]
\centering
\includegraphics[width=0.9\linewidth]{example-image-b}
\caption{Pfft.}
\label{fig:Pfft2}
\end{figure}
\lipsum[5-10]
\end{document}