我运行了awk -F= '$20+0 >= 99 && $31+0 >= 50' sample.txt，但没有找到以下文件的任何命中：
chr15_pilon_pilon.g1.t1 XP_016515887.1 XP_016515887.1 PREDICTED: uncharacterized protein LOC107832536 [Nicotiana tabacum] 2.7e-36 161.4 407 95 200 1 94 88 181 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAEMRMLRWMCGLTRLDKIKNEVIREKVGVAPVDEKMREARLMWFGHVRRRSPDAPVRSCERLAVVGASRGRGRPKRYWGEVIRQDMAKMRI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MNFHLQDGIEKGLVENDKKDHGEDERNDHEQDGRNDHVEVEPLAMTHETWKTELQVCLKGKFYKAVVRPAMLYGVECWPVKNSHVQRMKVAEMRMLRWMCGLTRLDKIKNEVIREKVGVAPVDEKMREARLMWFGHVRRRSPDAPVRSCERLAVVGASRGRGRPKRYWGEVIRQDMAKMRISEDMTLDRKVWRSSIRVVG 161.4 407 94 80.9 76 18 85 0 0 90.4 0 14HL20ED6TR1RM3NH11NS4EAIV2GARS1DG5KR4LV4RMTARK1QRVI 80.9 2.7e-36 98.9
chr15_pilon_pilon.g1.t1 XP_016492639.1 XP_016492639.1 PREDICTED: uncharacterized protein LOC107812125 [Nicotiana tabacum] 2.3e-35 158.3 399 95 316 1 94 188 281 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAETRMLKWMCGLTRLDKIKNEVIREKVGVAPVDEKMREARLRWFEHVRRRSPDAPVRSCERLTVVGARRGRGRPKRYWGEVITQDMARMRI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MTSISVNQFGFIPGRSTTEAIHLVRRLVEQFRDKKKDLHMVFIDLEKAYDKVPREVLWRCLEAKSVPEAYIRVIKDMYDGVKTRVKTVSGDSNHFPVVMGLDQGSALSPLLSKTDHLECKFSAEPSEVGRDMKIGSQVIAKRRLALGVLCDKKVPQKLKGKFYRAVVRPAMLYEAECWPVKNSHVQRMKVAETRMLKWMCGLTRLDKIKNEVIREKVGVAPVDEKMREARLRWFEHVRRRSPDAPVRSCERLTVVGARRGRGRPKRYWGEVITQDMARMRIFEDMALDRKVWRYQGCRIGASRIFRTPFPGLGGAW 158.3 399 94 79.8 75 19 84 0 0 89.4 0 5MT3RK4HL20ED6TR4GENH11NS4ETIV2GA2DG5KR4LV1RT2RMTA2QRVI 79.8 2.3e-35 98.9
chr15_pilon_pilon.g1.t1 XP_016503695.1 XP_016503695.1 PREDICTED: uncharacterized protein LOC107821758, partial [Nicotiana tabacum] 8.8e-35 156.4 394 95 205 1 94 93 186 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAETRMLRWMCGFTRLDKIRNEVIREKVGVASADEKLREARLRWFGHVRRRSPDAPVRSCERLEVVGASRGRGRPKKYWGEVIRHDMTSMWI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* SKTEYLECKFSAESSKEEVGRVVKLGSQAIAQRDIFKYLGLGQGGRNGGLHQESCVTRKCQKLKGKYYRAVVRPSMLYGAECWPVKKSHVQRMKVAETRMLRWMCGFTRLDKIRNEVIREKVGVASADEKLREARLRWFGHVRRRSPDAPVRSCERLEVVGASRGRGRPKKYWGEVIRHDMTSMWISEDMALDRRVWRSNIRVVG 156.4 394 94 78.7 74 20 82 0 0 87.2 0 5MT8HF6KR11PSVAED2ML3TR5NH11NS5IV2GARS1DG10LV2QH1RM1RS1QWVI 78.7 8.8e-35 98.9
chr15_pilon_pilon.g1.t1 OIT34550.1 OIT34550.1 putative ubiquitin-like-specific protease 2b [Nicotiana attenuata] 1.1e-34 156.0 393 95 574 1 94 175 268 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAEMRMLRWMCGHTRIDKIRNGDIREKVGVAPMEDKMREARLRWFGHIQRRSTDAPVRRCERLAVVGTRRGRGRPKKYWGEVIGQDMTRLRI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MCHIPYFSPFLFALVMDAITHHIQGEVPWCMLFADDIVLIDETQRGVNERLEVWRHALESKDFRLSRTKTEHLECKFGAEPTEAGVEVRLDSQVIPKRGSFKYLGSVIQGIGEIDEDVTHRIGVGWMKWRLATGVLCDKKVPPSLKGKFYRAVVRPAMLYGTECWPVKNSHIQKMKVAEMRMLRWMCGHTRIDKIRNGDIREKVGVAPMEDKMREARLRWFGHIQRRSTDAPVRRCERLAVVGTRRGRGRPKKYWGEVIGQDMTRLRITEDMALDRELWRSSIKVVGFGKKGTDEELPSQSRSTEHPRTCQKRKSKKVAAALDSAVLLRRSARLRGLSNKRNGKSDGKLNSVDFDCYLENIWRELPEDKKSSFTYLESMWFYLYTTKLFKAKVLRWIKGLDIFSKKYVFVPIVLWDHWCLLIFCNLGGSLESESKTPCMLLLDSLHMAGPSPYESEIRKFVLDIFKNEERPESQQLIKKIKLLIPKVPQQTNGTDCGKFALYFISLFLESAPENFSISEGYPYFMKRDWFTPDQLESFWLELPTLTKDSSEDMKNTAVSGESVSGSEDAIIYLV 156.0 393 94 76.6 72 22 84 0 0 89.4 0 17LI3KR1EGVD9VM1ED5TR5NHVIRQ3PT5NR4EAIV2GT2DG10LV1RG2RM2MLQRVI 76.6 1.1e-34 98.9
chr15_pilon_pilon.g1.t1 XP_016512735.1 XP_016512735.1 PREDICTED: uncharacterized protein LOC107829780 [Nicotiana tabacum] 2.0e-34 155.2 391 95 259 1 94 133 226 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAEIRMLRWMCGLTRLDKIKNEVIREKVGVAPVDEKMREARLRWFGHMRRRSPDAPVRICEWLAVVGVRRGRGRPKKYWGEVIRHDMDSMRI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MRVGVNERLEVWRQTLDSKGFRIRRPKTEYLECKFSAEASEVGRKVKIGSQVIAKKDCFKYLGSLIQGDGEIDGDVTHLIGAGWSKLSLVSRVLCDKKVPQKLKGEFYRAVVRPTMLYGAECWPVKKSHVQRMKVAEIRMLRWMCGLTRLDKIKNEVIREKVGVAPVDEKMREARLRWFGHMRRRSPDAPVRICEWLAVVGVRRGRGRPKKYWGEVIRHDMDSMRISEDMALDRITWRSSIRVVGQRHLEFLALLYRDG 155.2 391 94 79.8 75 19 83 0 0 88.3 0 5MI8HL20ED6TR5NHVM10NI2RW1EAIV2GV2DG10LV2QH1RMTDRS1QRVI 79.8 2.0e-34 98.9
chr15_pilon_pilon.g1.t1 XP_009783549.1 XP_009783549.1 PREDICTED: uncharacterized protein LOC104232135 [Nicotiana sylvestris] 3.3e-34 154.5 389 95 232 2 94 121 213 KVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV KVAEMRMLRWMCGHTRLDKIRNEDIRERVSVAPVDDKMREARFRWFGHVRRRSLDAPVRRCERLTLAGTRRGRGRPKKYWGEVIRQDMARLQI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MRRDAALIGDWRTKTEYVECKFSDVTGEADVEVRLDSQVILKRESFKYLGSIIQGDGEINRDVTHRIGVEWMKWRLAFGVLCDKNVSQKLKGKFYKVVVRPTMLYGVECWPVKNSHIQKIKVAEMRMLRWMCGHTRLDKIRNEDIRERVSVAPVDDKMREARFRWFGHVRRRSLDAPVRRCERLTLAGTRRGRGRPKKYWGEVIRQDMARLQISEDMALDRNMWRSNIRVVG 154.5 389 93 77.4 72 21 81 0 0 87.1 0 20KR2VD3KR1GS4EDED5TRLF4NH5PL5NR4ETILVA1GT2DG10LV4RMTA1ML1VI 77.4 3.3e-34 97.9
chr15_pilon_pilon.g1.t1 XP_018627647.1 XP_018627647.1 uncharacterized protein LOC104100193 [Nicotiana tomentosiformis] 9.7e-34 152.9 385 95 161 1 94 49 142 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAEMRMLRWMCGHTRMDKIRNEDIREKVGVAPMEDKMREVRLRWFGHIQRRSTDAPVRRCERLAVVGTRRGRGRPKKYWEEVIRDDMARLRI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MKWRLASGVLCDKKVPPLLKGKFYRAVVRPAMLYGTECWPVKNSHIQKMKVAEMRMLRWMCGHTRMDKIRNEDIREKVGVAPMEDKMREVRLRWFGHIQRRSTDAPVRRCERLAVVGTRRGRGRPKKYWEEVIRDDMARLRITEDMALDRELWRSSIKVVG 152.9 385 94 74.5 70 24 82 0 0 87.2 0 17LM3KR2VD9VM1ED4AVTR5NHVIRQ3PT5NR4EAIV2GT2DG8GE1LV2QD1RMTA1MLQRVI 74.5 9.7e-34 98.9
chr15_pilon_pilon.g1.t1 XP_016478590.1 XP_016478590.1 PREDICTED: uncharacterized protein LOC107799961 [Nicotiana tabacum] 1.3e-33 152.5 384 95 228 1 94 116 209 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAEMRMLRWMCRLTRLDKIKNEVIRQKVEMAPVDEKMREARLRWFGHVRRRSPDAPVRSCERLAVVGMRRGRGRPKRCWGEVIRHDMARMRV MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MVGVNEMLDVWRQTLESKGFKISRAKTEYLKCKFSAEPSEVGRDVKIGSQRDGEIDGDVTHRIGAGWSKWRLASGVLCDKKVPQKLKGKFYRAVVRPAILYRAECWPVKNSHVQRMKVAEMRMLRWMCRLTRLDKIKNEVIRQKVEMAPVDEKMREARLRWFGHVRRRSPDAPVRSCERLAVVGMRRGRGRPKRCWGEVIRHDMARMRVSEDMTLDRKVWRSSIRVVG 152.5 384 94 78.7 74 20 83 0 0 88.3 0 13GRHL12EQ2GEVM3ED6TR5NH11NS4EAIV2GM2DG5KRYC3LV2QH1RMTA2QR1 78.7 1.3e-33 98.9
chr15_pilon_pilon.g1.t1 XP_019244552.1 XP_019244552.1 PREDICTED: uncharacterized protein LOC109224430 [Nicotiana attenuata] 1.7e-33 152.1 383 95 208 1 94 49 142 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKAAEMSMLRWMCGHTRMDKVRNEGIREKVGVAPIDDNMREARLRWFGHVQRRSLDAPVRRCERLALVGTRRGRGRPKKYWGEVIRRDMARLQI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MKWRLASGVLCDKKVPLLLKGKFYRAVVRPAMLYGAECWPVKNSHTKKMKAAEMSMLRWMCGHTRMDKVRNEGIREKVGVAPIDDNMREARLRWFGHVQRRSLDAPVRRCERLALVGTRRGRGRPKKYWGEVIRRDMARLQISEDMALDRKLWRSSIKVVGGIVEMIVRVFARDLSVLLLLLIFGDKASFIYRGLRISETRQDNYLCR 152.1 383 94 73.4 69 25 82 0 0 87.2 0 2VA3RS10LM2IVKR2VG9VIEDEDKN4TR5NH1RQ3PL5NR4EAIL2GT2DG10LV2QR1RMTA1ML1VI 73.4 1.7e-33 98.9
chr15_pilon_pilon.g1.t1 OIT28878.1 OIT28878.1 dead-box atp-dependent rna helicase 32 [Nicotiana attenuata] 8.2e-33 149.8 377 95 1094 1 85 392 476 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELI MKVAEMRMLRWMCGLTRLDKIRNEVIREKVGVAPVDEKMREARLRWYGHVRRKSPDAPVRSCERLAVVGASRGRGRPKRYWGEVL MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MRKHTPKSRKAKIQSRLSEVHEIELLEAWIESGKPESGSNPLSLEPLPHKAPIGRLPDGSFSRYAGCDRFSQLPVSKKTKDGLIDCKFKTMTDIQRASLPHSLCGRDILGAAKTGSGKTLAFVIPFGFMPDRSTTEAIHLVRRLVEHFRDKKKDLHMVFIDLEKAYDKVPREVLWRCLEAKSVPEAYIRVIKDMYDGAKTRVRTVGGDSDHFPVVMGLHQGFALSPLLFALVMDAITCHIQGDVPWCMLFADDIVLIDETRVGGNERLEVWRQTLESKGFKLSRAKTEYLECKFSAEPSEVGRDVKLGSKVIAKRDNFRYLGSVIQGDGEIDGDVTHHIGEGWSKWRLASGVLCDKKVPQKLKGKSYRAVVRPAMLYGAECWPVKNSHVQRMKVAEMRMLRWMCGLTRLDKIRNEVIREKVGVAPVDEKMREARLRWYGHVRRKSPDAPVRSCERLAVVGASRGRGRPKRYWGEVLEKLYKARWGEEDGVGCIIMSPTRELAGQLFDVLKSVGKHHGFSAGLLIGGRKDVDTEKEHVNSLNILVCTPGRLLQHMDETPNFDCSQLQVLVLDEADRILDVGFKRELNAIISQLPKHRQTLLFSATQTKSVQDLARLSLKDPEYLGVHEESDTATPNRLQQTAMLVPLDKKLDMLWSFIKAHLNSRILVFLSSCKQVKFVFEAFKKLRPGIPLKCLHGRMKQDRRMGIYSQFCEQRSVLFSTDVASRGLDFNKAVDWVVQVDCPEDCAAYIHRVGRTARYLSGGRSLLFVMPSEMKMLEKLEEKKIPLRVIKANEKRIQSVSDLLASLLVKYPDLQHLSQRAFVTYLKSIHKQRDKEIFDVTKLPIDEFSASLGLPMTPKIRFLKQKLKGKTVSEALSLLPDDTSNDNLLELPIRKPDTGKSEGEEVEEDLLLAKETQEVGELKINSKGDDMLATRVLKKKKLKINVHRPVGTRVVFDEEGNTLPPLARLADTSSGADSVQLNKEKVNQRYAELRKNLKLADKEDKDLDRKRLQEKRIKEKMKYKRGREEEEDEELSESDGELPGGRVNKKTKIFDSDDDDGDKSKDMAKEGIAADAISVAEQEELALKLLSSMNS 149.8 377 85 81.2 69 16 79 0 0 92.9 0 14HL6KR13ED6TR3FY1NH3RK7NS4EAIV2GARS1DG5KR4LVIL 81.2 8.2e-33 89.5
chr15_pilon_pilon.g1.t1 XP_018634600.1 XP_018634600.1 uncharacterized protein LOC108949236 [Nicotiana tomentosiformis] 8.2e-33 149.8 377 95 202 1 94 90 183 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAEMRMLRWMCGHTRLDKIRNVEFREKVGMAPMDDKMREARLRWFGHVRRRSIDAPVRGCETLALAGTRGGRGRPKKYWGEVIRQDMARLQI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MDVRLDSQAIPKRGNFKYLESIIQGDGEIDEDVTHRIGVGRMKWGLASGVLFDKKVPPKLKGKFYRAVVRPAMLYGAECWAAKNSHTQKMKVAEMRMLRWMCGHTRLDKIRNVEFREKVGMAPMDDKMREARLRWFGHVRRRSIDAPVRGCETLALAGTRGGRGRPKKYWGEVIRQDMARLQISEDMALDREGWRSRIRVVG 149.8 377 94 74.5 70 24 80 0 0 85.1 0 21KR1EVVEIF5VM2VMEDED5TR5NH5PI5NG2RT1EAILVA1GT1RGDG10LV4RMTA1ML1VI 74.5 8.2e-33 98.9
chr15_pilon_pilon.g1.t1 XP_019238436.1 XP_019238436.1 PREDICTED: uncharacterized protein LOC109218522 [Nicotiana attenuata] 3.1e-32 147.9 372 95 161 1 94 49 142 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAKMRMLRWMCGHTRIDKIRNEVIRDKVGVAPVDDKMRELRLRWFGHVQRRSTDAPVRRCERLAVAGLRRGRGRLKKYWGEMITQDMALLQL MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MNWGLASGVLCDKKVPPRLKGKFYRVVVRPTMLYVAECWPIKNFHFQRMKVAKMRMLRWMCGHTRIDKIRNEVIRDKVGVAPVDDKMRELRLRWFGHVQRRSTDAPVRRCERLAVAGLRRGRGRLKKYWGEMITQDMALLQLTEDMIQDRRAWKSKIRVVG 147.9 372 94 73.4 69 25 81 0 0 86.2 0 4EK12LI3KR5ED7EDED4ALTR5NH1RQ3PT5NR4EAIVVA1GL2DG3PL6LM1RT2RMTARLML1VL 73.4 3.1e-32 98.9
chr15_pilon_pilon.g1.t1 XP_016515386.1;XP_033517730.1 XP_016515386.1 PREDICTED: uncharacterized protein LOC107832086 [Nicotiana tabacum] 2.0e-31 145.2 365 95 599 1 94 70 163 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVVEMRMLRWMCGHTRRDKIRNEVIRDKVGVAPLEAKMRELRLRWFGHVQRKSTDAPVSRCERLAVAGLRRGRGRPKKYWAEVIRQDMALLQL MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MMSKGFKLSRAKTEYLECKFSDGTHEADVKVKLDTQVIPRRSSFKYLGSIIRGNGEIDDEVSHRIGAGRMKVVEMRMLRWMCGHTRRDKIRNEVIRDKVGVAPLEAKMRELRLRWFGHVQRKSTDAPVSRCERLAVAGLRRGRGRPKKYWAEVIRQDMALLQLTEDMIQDRRVWSSKIKVKAWVAEKDAEALRCQKLLFEEEEAAQKKQAELLERKKVKKLRQKEQKAKEQSIEEKGILEAPANIPDVSVGISTCLEMVQFSSNVDTSVESQYDLCHQHLDSVKVQNLEPRPVSANSRRRFANSQLQAPKSQRFGRNGGFHNNQNHQALKAEPIQKHKDSGTPVNSCMIWTRKVRVESDDTSILEVQKEDIDQKQRNSEVIIGSISVPVKDCSTQQQGRDDFGSTELRKKCNAVEKPAKHDALQIGSNRAAAKLWRPVRHTVGRQDPEEEGVMSSKFDDGTSLNENSLQSCPVDSSGSRKNCQVPDGNAHQGLGYSSIAAKAFLAQRWREAIAGDHVRLVLSPDTESSERPEVPSSSSEAAPASDSGEHGVVSRADTELTKNEVLTSSSSGSIKVKYRPKPEKGVKTMYIPKQKNHHLG 145.2 365 94 72.3 68 26 78 0 0 83.0 0 3AV13LR3KR5ED6VL1EA4ALTR5NH1RQ1RK1PT4RSNR4EAIVVA1GL2DG8GA1LV4RMTARLML1VL 72.3 2.0e-31 98.9
chr15_pilon_pilon.g1.t1 XP_033510858.1 XP_033510858.1 uncharacterized protein LOC117275662 [Nicotiana tomentosiformis] 2.6e-31 144.8 364 95 258 1 94 160 253 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MNVTEMRILRWMCGHIRLDKVRNEDIREKVGVASMEDKMHEARLRWFGHVRRRSLDAPVRRCEQLALAGRRRGRGRPKKYWGDVIRQDMARLQL MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MDTPTRHIQGEVPWCMLFADDIVLIDETRGGVNEKLEVCRQTLESKGFELSKTKTEYLECKFNVVTWGANIAVRLDSQVIPNRGSFKYLRSVIQGSGEIGEDVTHRIGAGWAKWRLASGVLCDKNVPPKLKGQFYKAAIRLGMLYGAECWPVKNSHIQKMNVTEMRILRWMCGHIRLDKVRNEDIREKVGVASMEDKMHEARLRWFGHVRRRSLDAPVRRCEQLALAGRRRGRGRPKKYWGDVIRQDMARLQLTWPLT 144.8 364 94 71.3 67 27 79 0 0 84.0 0 1KN1AT3MI7TI4IVKR2VD8PSVM1ED2RH2TR5NH5PL5NR2RQ1EAILVA1GR2DG9EDLV4RMTA1ML1VL 71.3 2.6e-31 98.9
chr15_pilon_pilon.g1.t1 OIT40319.1 OIT40319.1 exocyst complex component sec6 [Nicotiana attenuata] 2.6e-31 144.8 364 95 1287 1 83 993 1075 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGE MKVAEMRMLRWMCGLTRLDKIRNEVIREKVGVALVEEKMREARLRWYGHVRRRSPDAPVRSCEQLAVVGASRGRGRPKRYWGE MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MMAEDLGVEAKEASVREVAKLLPLPELLQSISSIKADYIARQQANDAQLSTMVAEQVEQAQAGLQSLNSSQKTINQLRENFLSIEKLCQECQNLIENHDQIKLLSNTRNNLNTTLKDVEGMMSISVEASEARDSLRDDKELINTYERLTALDGKRRFALAAAGSHKEEVGRLREYFEDVDQTWETFEKTLWAHIANFSKLAKESPQTLVRAIRVVEMQEILDQQLAEEAAEAEGGGAMASIANPRRNAKKTPSSKNLTQQKLKVQGKGYKDKCYESIRKSVEARFDKLLNEEELKTAIEEARVMGEELGEIYDYVAPCFPPRYEVFQLMVNLYTERFVQWLRKLSDQANNMTNIEILKVTGWVVDYQESLIGLGVDDSLAQVCSESGAMDPLMNAYVERMQATTKKWYLNILEADKVQPPKKTDDGKLYTPAAVDLFRILGEQVQIVRENSTDVMLYRIALALIQVMIDFQAAERKRLEEPASEIGLESLCAMINNNLRCYDLAMELSSSTLEALPQNYAEQVNFEDTCKGFLEVAKYGEADAMWSETANLVREAARDVLGVAKGYSGRHQGDWWWNDVVQGKVEAKKAAFAKLAGSSSEEERRANRESYKVARKEAKLAVTEAKNAAFGRMYEELGEKGGDRKLFRLAKVRERKARDLDQVRCIKDEDGRVLMGESQVKQRWQAYFRDLLNEEGDRDVELGELGNSEFHRDLGYCRRIRVEEVVGALRRMSRGRATGPDEIPVEFWKCIGRSGVEWLSRLFNVIFRTKRMPEEWRDKKKDLHMVFIDLEKAFDKVPREVLWRCREAKSVPEAYIRVIKDMYDGAKTRVRTVGGDSDHFSVVMGLHQGSALSPLLFALVMDAITRHIQRDVPWCMLFADDIVLLDETRVGVNERLEVWRKTLESKGFKLSRAKTEYLECKFSAEPSEGDGEIDGDVTHRIGAGWWKWRLASGVLCDKKVPQKLKGKFYRVVVRPAMLYGAECWPVKNSHVQRMKVAEMRMLRWMCGLTRLDKIRNEVIREKVGVALVEEKMREARLRWYGHVRRRSPDAPVRSCEQLAVVGASRGRGRPKRYWGEEAVHQTVSVIFEDPGVEELLVKLYQKDWLEGQVTEFLVATFSDYFTDVKMYIEERSFRRFVEACLEETVVVYVDHLLTQKNYIKEETIERMRLDEEVLMDFFREYISVSKVENRVRILSDLRELASSESPDSFTLVYTNILEQQPDCPPEVVEKIVGLREGIPRKDAKEVVQECKEIYEHSLVDGNPPKTGFVFPRVKCLSAAKHSIWRKLT 144.8 364 83 83.1 69 14 76 0 0 91.6 0 14HL6KR11PL8TR3FY1NH11NS2RQ1EAIV2GARS1DG5KR4 83.1 2.6e-31 87.4
chr15_pilon_pilon.g1.t1 XP_009775799.1 XP_009775799.1 PREDICTED: uncharacterized protein LOC104225652 [Nicotiana sylvestris] 3.5e-31 144.4 363 95 265 2 94 146 238 KVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV KVAEIRMLKWMCEHTRLDKIRNEDIRERVCVAPVDDKMREAQLRWFVHVRKRSLDAPVRRCERLTLAGTRRGRGRPKKYWGEVIRQDMARLQI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MLFADDIVLIDETRGGVNGRLEVWRQALESKGFKLSRTKTEYVECKFSDVTEEVDVEVRLGSQVILKRESFKYLRLIIHGDGEIDGDVTHRIGVGWMKWILASGVLCDKNVPPKLKSKFYRAVVRPAMLYGAEFWPVKNPHIQKIKVAEIRMLKWMCEHTRLDKIRNEDIRERVCVAPVDDKMREAQLRWFVHVRKRSLDAPVRRCERLTLAGTRRGRGRPKKYWGEVIRQDMARLQISEDMALDSYLLLYASNGAVSNGGIEEV 144.4 363 93 73.1 68 25 80 0 0 86.0 0 4MI3RK3GE7KR2VD3KR1GC4EDED5TQ4GVNH2RK2PL5NR4ETILVA1GT2DG10LV4RMTA1ML1VI 73.1 3.5e-31 97.9
chr15_pilon_pilon.g1.t1 OIT33527.1 OIT33527.1 hypothetical protein A4A49_14946 [Nicotiana attenuata] 5.9e-31 143.7 361 95 885 1 94 116 209 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MRVAEMRMLRWMCGNTRMDKIRNVDIREKVLVAPIDDKMREARLRWFGHVQRRSPDAPVRMCEQLVMEGTRRGRRRPKKYWEEVIRQDMTRLQI MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MSLDKSTTTKTEYMECKFTAEPGQVGVDMRLESQVIPSRGNFKYLGSVIHGGGEIDEDVTHRIGVGWMKWRLASGVLCDKRLLPLLKGKFYKAVVRPAMMYGAECWPVKNLHIQKMRVAEMRMLRWMCGNTRMDKIRNVDIREKVLVAPIDDKMREARLRWFGHVQRRSPDAPVRMCEQLVMEGTRRGRRRPKKYWEEVIRQDMTRLQIFEDMTLDRKMWRSSIRVIGLAHHDNYHEFCRLLGRFKVNYQLSELVNVESYGDWIRLVAEFTLRSLQSWQWASSSVYYLLGLWSRLVSSVPYLKGDTPSLLSEFVPEIVKSFITSRFGSFQGEISDLSENPLDNVDLLQDQLDCLPYLCRFQYESCSSYIMQITDPLLQMYMESADPQVLTVVETKFAWIVHIIAAIVKTKQLSGYSGESQEILDAELSARVLRLINVTDSGLRSQRYAETSKQRLDLAILVFFQNFRKSYVGDQAIHSSKQLYAKLAELLGLHDHMLILNVIVGKIATNLKFYGESEEVISQTLNLMLEMASGYMTAKLLVKLDTTQLIISNRNREEFPFLGDFRCSRSRTTFYYIIGLLIFMEDSFLKFKASMDPLLQVLLSLELILDALFRTDDVKQALIGLMRDLRGIAMATSSRRTYGFLFDWLYPAHIPLLLKAIRIWADTPEVTTPLLKFIAEFVLNKSQRLNFEISSPNGILLFREVSKLIVAYGSRILSLPSHVDMYQFKYKGIWISLTILSRALAGNYVNFGVFEIYGDRALADAFDISMRMALSIPLADILAYRKLSGAYFTFLEIMMKNQIHLILNLDSSSFIFIAGSLESGLKVLDANIKSQCASAVDNLATFYFDHITAGESPTNPEALNLAQHLADCPNIFLEVSSTALFI 143.7 361 94 72.3 68 26 82 0 0 87.2 0 1KR12HN2LM3KR1EVVD5GL3VIEDED5TR5NH1RQ9NM2RQ1EVIMVE1GT2DG1GR6GE1LV4RM2ML1VI 72.3 5.9e-31 98.9
chr15_pilon_pilon.g1.t1 XP_033512047.1 XP_033512047.1 uncharacterized protein LOC104096328 [Nicotiana tomentosiformis] 5.9e-31 143.7 361 95 413 1 94 226 319 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAEMSMLRWMCGHTRLDRIMSEVIRDKVGVAPVEANMREARLRWFGHVKRRSTDAPVRRCERLALGGERRGRGRPKKSWGEVIRRDMEQLEL MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MNSDIFQFLCFNLEKAYDKVPREVLWRCLEAKGVPVVYIRVIQDMYDGAKTQVRTAGGDSDYFPVEMGLHQGSALSPFLFSLAMDSLTLRIDETRGGVNERLEVWRHTLESKGFKLNKTKTEYFECKFSGVTQEADGDVRLDTQVIPRRESFKYLGSIIQKNEKIDEDVTHRIRAGWMKWRLASGVLCDKNVSLRLKGKFYKVVVRPTMLYGAECWPVKKSHVQQMKVAEMSMLRWMCGHTRLDRIMSEVIRDKVGVAPVEANMREARLRWFGHVKRRSTDAPVRRCERLALGGERRGRGRPKKSWGEVIRRDMEQLELTEDMTLDRRVWKLKIRVKGLPSPSLAEGLSETVSLPLQGRGKAALAVCFLLLGCSTDVYNLNELIVNSYQLPSKNDNNLSSSTSHESSISNFSI 143.7 361 94 72.3 68 26 80 0 0 85.1 0 6RS12KR1KMNS4ED8EAKN4TR5NH1RK3PT5NR4EAILVG1GE2DG6YS3LV2QR1RMTERQMLQEVL 72.3 5.9e-31 98.9
chr15_pilon_pilon.g1.t1 XP_033511396.1 XP_033511396.1 uncharacterized protein LOC117276177 [Nicotiana tomentosiformis] 7.7e-31 143.3 360 95 109 1 88 18 105 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQD MKVAEMRMLRWICGHTRRDKIKNEVIRDKAGVAPVEEKMRELRLRWFGYVQRRSTDAPVKRCERLAVAGLRRGRGRANKFWGEVIRQD MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MLYGAEYWPVKNSHVRGMKVAEMRMLRWICGHTRRDKIKNEVIRDKAGVAPVEEKMRELRLRWFGYVQRRSTDAPVKRCERLAVAGLRRGRGRANKFWGEVIRQDMALL 143.3 360 88 77.3 68 20 75 0 0 85.2 0 11MI5LR9ED1VA11ALTR5NY1RQ3PT4RKNR4EAIVVA1GL2DG3PAKN1YF3LV4 77.3 7.7e-31 92.6
chr15_pilon_pilon.g1.t1 KAF3643615.1 KAF3643615.1 putative pre-mRNA-processing factor 6-like [Capsicum annuum] 1.0e-30 142.9 359 95 209 1 94 97 190 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV MKVAEMRMLRWMCGLTRGDRVRNETIREKVGVTPVECKMREARLRWFGHVKRRAMDAPVRRCERLALDGFRRGRGRPKKYWGEVIRRDMEQLQL MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV* MWKKVKVECNKGCLVLIGGDSNVRKSTLLLQINGEIDEDVSHRIGTGWMKWKLASGVLCDKKVPPKLKGKFYRVVVRPAMLYGAECWPVKNSHIQKMKVAEMRMLRWMCGLTRGDRVRNETIREKVGVTPVECKMREARLRWFGHVKRRAMDAPVRRCERLALDGFRRGRGRPKKYWGEVIRRDMEQLQLTEDMTLDRKVWRTRIRAED 142.9 359 94 72.3 68 26 80 0 0 85.1 0 14HL2LG1KRIVKR2VT7AT3EC5TR5NH1RK2SAPM5NR4EAILVD1GF2DG10LV2QR1RMTERQML1VL 72.3 1.0e-30 98.9
我错过了什么？
答案1
正如 @steeldriver 已经评论的那样，数据中的字段似乎没有用 分隔=，因此FS使用此分配将（字段分隔符）设置为此值-F=将导致随机输出。
此外，即使您使用默认的字段分隔符（空格），我也看不到您想要的字段20和字段。31
如何查看数据、调试、决定并形成命令
用长行标识此文件中所需的字段是程序的工作。首先使用 awk 看看这些行有什么。首先更好地查看和理解这个文件，然后决定要执行的命令是什么。对于这些长线来说，手动（用肉眼）计算田地是很困难的。
例如，仅检查第一行。跑步
awk 'NR==1 {for (i=1;i<=NF;i++) print i, $i}' file
将为您提供所有内容的字段索引和值：
1 chr15_pilon_pilon.g1.t1
2 XP_016515887.1
3 XP_016515887.1
4 PREDICTED:
5 uncharacterized
6 protein
7 LOC107832536
8 [Nicotiana
9 tabacum]
10 2.7e-36
11 161.4
12 407
13 95
14 200
15 1
16 94
17 88
18 181
19 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV
20 MKVAEMRMLRWMCGLTRLDKIKNEVIREKVGVAPVDEKMREARLMWFGHVRRRSPDAPVRSCERLAVVGASRGRGRPKRYWGEVIRQDMAKMRI
21 MKVAEMRMLRWMCGHTRLDKIKNEVIREKVGVAPVEEKMREATLRWFGNVRRRSPDAPVRNCERLEIVGGRRDRGRPKKYWGELIRQDRTRMQV*
22 MNFHLQDGIEKGLVENDKKDHGEDERNDHEQDGRNDHVEVEPLAMTHETWKTELQVCLKGKFYKAVVRPAMLYGVECWPVKNSHVQRMKVAEMRMLRWMCGLTRLDKIKNEVIREKVGVAPVDEKMREARLMWFGHVRRRSPDAPVRSCERLAVVGASRGRGRPKRYWGEVIRQDMAKMRISEDMTLDRKVWRSSIRVVG
23 161.4
24 407
25 94
26 80.9
27 76
28 18
29 85
30 0
31 0
32 90.4
33 0
34 14HL20ED6TR1RM3NH11NS4EAIV2GARS1DG5KR4LV4RMTARK1QRVI
35 80.9
36 2.7e-36
37 98.9
或者相同，仅获取数值的字段索引和值：
awk 'NR==1 {for (i=1;i<=NF;i++) if ($i==$i+0) print i, $i}' file
10 2.7e-36
11 161.4
12 407
13 95
14 200
15 1
16 94
17 88
18 181
23 161.4
24 407
25 94
26 80.9
27 76
28 18
29 85
30 0
31 0
32 90.4
33 0
35 80.9
36 2.7e-36
37 98.9
请注意，测试 for$i+0 == $i是标准的 awk 习惯用法，用于测试字段是否为数值。
现在您可以看到您的数据。如果需要，您可以打印一些字段以获得更多行。您可以根据需要决定并形成您的命令。