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在 Linux Mint 上激活 BWA 时出现“内联失败”错误

tag-icon • tag-icon linux bash linux-mint
在 Linux Mint 上激活 BWA 时出现“内联失败”错误

我目前正在尝试在我的 Virtualbox Linux Mint 64 位上激活最新的 bwa 包。我已按照 README 文件中的说明进行操作：

## Getting started

    git clone https://github.com/lh3/bwa.git
    cd bwa; make

但是当我输入“make”并按回车键时，出现以下错误：

ksw.c: In function ‘ksw_u8’:
ksw.c:131:7: warning: SSE vector return without SSE enabled changes the ABI [-Wpsabi]
  zero = _mm_set1_epi32(0);
  ~~~~~^~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:654:1: error: inlining failed in call to always_inline ‘_mm_set1_epi8’: target specific option mismatch
 _mm_set1_epi8 (char __A)
 ^~~~~~~~~~~~~
ksw.c:136:8: note: called from here
  shift = _mm_set1_epi8(q->shift);
  ~~~~~~^~~~~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:654:1: error: inlining failed in call to always_inline ‘_mm_set1_epi8’: target specific option mismatch
 _mm_set1_epi8 (char __A)
 ^~~~~~~~~~~~~
ksw.c:135:8: note: called from here
  e_ins = _mm_set1_epi8(_e_ins);
  ~~~~~~^~~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:654:1: error: inlining failed in call to always_inline ‘_mm_set1_epi8’: target specific option mismatch
 _mm_set1_epi8 (char __A)
 ^~~~~~~~~~~~~
ksw.c:134:9: note: called from here
  oe_ins = _mm_set1_epi8(_o_ins + _e_ins);
  ~~~~~~~^~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:654:1: error: inlining failed in call to always_inline ‘_mm_set1_epi8’: target specific option mismatch
 _mm_set1_epi8 (char __A)
 ^~~~~~~~~~~~~
ksw.c:133:8: note: called from here
  e_del = _mm_set1_epi8(_e_del);
  ~~~~~~^~~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:654:1: error: inlining failed in call to always_inline ‘_mm_set1_epi8’: target specific option mismatch
 _mm_set1_epi8 (char __A)
 ^~~~~~~~~~~~~
ksw.c:132:9: note: called from here
  oe_del = _mm_set1_epi8(_o_del + _e_del);
  ~~~~~~~^~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:642:1: error: inlining failed in call to always_inline ‘_mm_set1_epi32’: target specific option mismatch
 _mm_set1_epi32 (int __A)
 ^~~~~~~~~~~~~~
ksw.c:131:7: note: called from here
  zero = _mm_set1_epi32(0);
  ~~~~~^~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:713:1: error: inlining failed in call to always_inline ‘_mm_store_si128’: target specific option mismatch
 _mm_store_si128 (__m128i *__P, __m128i __B)
 ^~~~~~~~~~~~~~~
ksw.c:142:3: note: called from here
   _mm_store_si128(Hmax + i, zero);
   ^~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:713:1: error: inlining failed in call to always_inline ‘_mm_store_si128’: target specific option mismatch
 _mm_store_si128 (__m128i *__P, __m128i __B)
 ^~~~~~~~~~~~~~~
ksw.c:141:3: note: called from here
   _mm_store_si128(H0 + i, zero);
   ^~~~~~~~~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:713:1: error: inlining failed in call to always_inline ‘_mm_store_si128’: target specific option mismatch
 _mm_store_si128 (__m128i *__P, __m128i __B)
 ^~~~~~~~~~~~~~~
ksw.c:140:3: note: called from here
   _mm_store_si128(E + i, zero);
   ^~~~~~~~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:1191:1: error: inlining failed in call to always_inline ‘_mm_slli_si128’: target specific option mismatch
 _mm_slli_si128 (__m128i __A, const int __N)
 ^~~~~~~~~~~~~~
ksw.c:149:5: note: called from here
   h = _mm_slli_si128(h, 1); // h=H(i-1,-1); << instead of >> because x64 is little-endian
   ~~^~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:695:1: error: inlining failed in call to always_inline ‘_mm_load_si128’: target specific option mismatch
 _mm_load_si128 (__m128i const *__P)
 ^~~~~~~~~~~~~~
ksw.c:148:5: note: called from here
   h = _mm_load_si128(H0 + slen - 1); // h={2,5,8,11,14,17,-1,-1} in the above example
   ~~^~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:695:1: error: inlining failed in call to always_inline ‘_mm_load_si128’: target specific option mismatch
 _mm_load_si128 (__m128i const *__P)
 ^~~~~~~~~~~~~~
ksw.c:174:6: note: called from here
    h = _mm_load_si128(H0 + j); // h=H'(i-1,j)
    ~~^~~~~~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:1377:1: error: inlining failed in call to always_inline ‘_mm_max_epu8’: target specific option mismatch
 _mm_max_epu8 (__m128i __A, __m128i __B)
 ^~~~~~~~~~~~
ksw.c:172:6: note: called from here
    f = _mm_max_epu8(f, t);
    ~~^~~~~~~~~~~~~~~~~~~~
In file included from ksw.c:29:0:
/usr/lib/gcc/i686-linux-gnu/7/include/emmintrin.h:1100:1: error: inlining failed in call to always_inline ‘_mm_subs_epu8’: target specific option mismatch
 _mm_subs_epu8 (__m128i __A, __m128i __B)
 ^~~~~~~~~~~~~
ksw.c:171:6: note: called from here
    t = _mm_subs_epu8(h, oe_ins); // h=H'(i,j) - o_ins - e_ins
    ~~^~~~~~~~~~~~~~~~~~~~~~~~~~

整个错误信息太长，无法粘贴，但我想你明白了。

答案1

只要运行apt-get update && apt-get upgrade make gcc zlib1g-dev就可以了。让我解释一下为什么它会这么想：

如果它没有帮助，而您只对这个确切案例的解决方案感兴趣，请跳到我的答案的末尾。

我通常使用 docker 解决构建问题，以便可以创建我的发行版的最小化环境。

您正在为 Mint 构建，它基本上是 Ubuntu 加上额外的功能。我假设您的版本是 Mint 19，它基于 Ubuntu 18.04，所以我运行docker run --name bwabuild -it --rm ubuntu:18.04。从现在开始，我在容器内运行所有提到的命令：

  • 我需要 git：apt-get update && apt-get install git
  • 我需要回购：git clone https://github.com/lh3/bwa.git && cd bwa
  • 我需要解析 makefile：apt-get install make

从现在起，我只是make一遍又一遍地运行，并始终处理我看到的第一个新错误。这些是我注意到的并且必须解决的问题：

  • 没有海湾合作委员会：apt-get install gcc
  • 没有 zlib.h：我在“真实”系统上搜索（它在）并在“真实”系统上运行apt-get install zlib1g-dev
    ，发现这是所需的包zlib.h/usr/include/zlib.hdpkg -S /usr/include/zlib.h

从这里开始，它可以正确构建（带有警告，但那只是因为代码“丑陋”）。我没有收到任何错误。所以现在我检查软件包版本：

  • dpkg -l make：4.1-9.1ubuntu1
  • dpkg -l gcc：4：7.4.0-1ubuntu2.3
  • dpkg -l zlib1g-dev：1：1.2.11.dfsg-0ubuntu2

我现在知道我需要哪些包以及版本应该是什么，所以我exit使用容器（因为我用它运行，所以它也会杀死它--rm）。

我在我的主机系统上更新了这 3 个包（巧合的是我也使用 Mint）并尝试以与您相同的方式进行构建，现在构建完美。

如果由于某种原因它仍然无法在您的系统上构建，您可以像我一样在 docker 容器中执行此操作。这肯定会起作用，因为环境与我的情况完全相同。
构建完成后（退出容器之前），使用第二个终端运行以将docker cp bwabuild:/root/bwa/bwa .bwa 程序从容器内复制到当前目录。

请注意：最后一种解决方案需要一定的工作才能构建它，但不一定能运行它。该程序是在假设某些库位于某些位置的情况下构建的。这些库在主机系统和容器中的位置可能不同（尽管这会让我感到惊讶，因为它们都基于 Ubuntu 18.04）

编辑：

我刚刚注意到这个程序也可以用 conda 安装：conda install -c bioconda bwa

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